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KMID : 0545120150250050732
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 5 p.732 ~ p.737
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In Silico Metagenomes Mining to Discover Novel Esterases with Industrial Application by Sequential Search Strategies
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Jorge Barriuso
Maria Jesus Martinez
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Abstract
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We present here an in silico search of fungal sterol-esterase/lipase and bacterial depolymerase sequences from environmental metagenomes. Both enzyme types contain the α/β-hydrolase protein fold. Analysis of DNA conserved motifs, protein homology search, phylogenetic analysis, and protein 3D modeling have been used, and the efficiency of these screening strategies is discussed. The presence of bacterial genes in the metagenomes was higher than those from fungi, and the sequencing depth of the metagenomes seemed to be crucial to allow finding enough diversity of enzyme sequences. As a result, a novel putative PHAdepolymerase is described.
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KEYWORD
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Sterol esterase, lipase, PHA-depolymerase, genome diversity
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